Protein Modification May Hold Key to Reversing Cellular Aging

Figure 4. Effect of 2-BP treatment on senescence markers. Levels of p-p53 (A), HMGB1 (B), and lamin B1 (C) were measured in cell extracts from VSMCs. Cells were either pretreated with 2-BP for 24 hours before doxorubicin (DOX) treatment or treated with 2-BP alone for the specified durations. Protein levels were assessed using Western blotting, with representative blots shown (the red line indicates where the blot was cut) (D). Data are presented as means ± SEM from N=3 biological replicates. Statistical significance was determined using one-way ANOVA; *p Read Entire Article
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